Research Article

Inhibition of the NLRP3 Inflammasome by a Quercus Serrata Extract and Isolation of the Component Compounds for the Treatment of Arthritis

Table 1

Analysis conditions for high-performance liquid chromatography (HPLC).

HPLC condition

Mobile phaseA: 0.2% acetic acid in H2O
B: Acetonitrile (ACN)
Flow rate: 1.0 mL/min
Injection vol.: 10.0 μL
Gradient profileTime (min)A (%)B (%)
0.0
10.0
35.0
55.0
59.0
95
75
50
0
0
5
25
50
100
100
HPLC instrument
Controller
Column
Detector
Waters 2695 Separations Module
Hector C18 HPLC Column (5 μm, 250 × 4.6 mm)
Waters 996 Photodiode Array Detector