Research Article
The Antitumor Effect of TPD52L2 Silencing on Oxaliplatin-Resistant Gastric Carcinoma Is Related to Endoplasmic Reticulum Stress In Vitro
Figure 1
Depiction of the expression of TPD52L2 in OXA-resistant gastric carcinoma cell lines. (a, b) MTT assay was employed for the evaluation of cell viability in cells, i.e., SGC-7901 and MGC-803 cells, and their OXA-resistant counterparts, i.e., SGC-7901-OXA and MGC-803-OXA, after culturing for two days with increasing concentrations of OXA. IC50 (μM) values of cells were examined. (c) The mRNA levels of TPD52L2 were identified via qRT-PCR and normalized to the levels of GAPDH that are expressed as fold-change relative to the parental cell lines. (d) The protein expression level of TPD52L2 was determined via immunoblotting with GAPDH as the loading control. (e) Quantification of TPD52L2 protein levels normalized to GAPDH. All values are indicated as mean ± SD of three replicates. value <0.05 and value <0.01 compared with the corresponding control cells.
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