The Antitumor Effect of TPD52L2 Silencing on Oxaliplatin-Resistant Gastric Carcinoma Is Related to Endoplasmic Reticulum Stress In Vitro

Figure 4

Effect of silencing of TPD52L2 on apoptosis and ER stress-associated proteins in OXA-resistant gastric carcinoma cell lines. (a) Protein expression levels of apoptosis-associated factors, i.e., cleaved PARP and cleaved caspase-3, in SGC-7901-OXA and MGC-803-OXA cells transfected with siCon or siTPD52L2 determined via immunoblotting. (b) The translational levels of apoptosis-associated factors were normalized to GAPDH. (c) Immunoblotting analysis of ER stress-associated proteins, i.e., GRP78, PERK, CHOP, and IRE1α. (d) Protein expression levels of ER stress-related factors were normalized to GAPDH. All values are shown as mean ± SD of three replicates. value <0.01 compared to the siCon group of each cell line.

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