Fu Fang Zhen Zhu Tiao Zhi Capsules Protect against Myocardial Ischemia by Inhibiting Cardiomyocyte Pyroptosis

Figure 3

FTZ inhibits pyroptosis in vitro. (a and b) Double-fl;uorescence staining with DAPI (blue) and TUNEL (green) and quantitative TUNEL data. Scale bar indicates 100 μm (right); n = 3. (c and d) Representative western blots (3 independent experiments) (left) and quantiflcation of western blots in NMCMs. β-actin was used as an internal control. (e) Quantitative RT-PCR for NLRP3, GSDMD, ASC, C-Caspase-1, and GSDMD-N. (f) The degree of cell insult was assessed using % LDH release. and vs. CTRL;^# and ^## vs. H₂O₂. (g) NLRP3 representative immunofluorescence images. Scale bar indicates 100 μm; n = 3.

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