Curcumin Prevents Diabetic Osteoporosis through Promoting Osteogenesis and Angiogenesis Coupling via NF-κB Signaling

Figure 3

Cur increased VEGF expression and secretion to improve the proangiogenic capacity of BMSCs in HG. (a)–(b) VEGF protein expression of BMSCs in each group were assessed using western blot. (c) Detection of VEGF concentration in conditioned medium from BMSCs in each group using ELISA. (d)–(j) Endothelial cells were incubated with Cur pretreated BMSC CM, supplemented with or without VEGF neutralizing antibodies. (d)–(e) Proliferation was determined by EDU555 staining. (f)–(g) Cell motility in each group was evaluated using the scratch wound assay. (h)–(j) Representative images and quantification of tube formation were assessed in each group. Data are presented as the mean ± SD from at least three independent experiments. and versus NG group. ^# and ^## versus HG group. ^& and ^&& versus CMHG + Cur group.