Curcumin Prevents Diabetic Osteoporosis through Promoting Osteogenesis and Angiogenesis Coupling via NF-κB Signaling

Figure 5

NF-κB signaling is involved in HG-inhibited osteogenic differentiation and proangiogenic ability of BMSCs. Western blot of p65 and p-p65 in BMSCs treated with HG or HG + Bay117082 (Bay) (a) and (b). Osteogenic differentiation of BMSCs treated with HG or HG + Bay were determined with (c) ALP staining and ALP activity assays, and (d) alizarin red staining and calcium deposition analysis. Endothelial cells stimulated with conditioned medium from BMSCs treated with HG or HG + Bay. (e) and (f) Endothelial cell motility in each group was evaluated using the scratch wound assay. (g) and (h) Representative images and quantification of tube formation were assessed in each group. (i) and (j) Expression of Runx2 and VEGF protein in each group by western blot. Data are presented as the mean ± SD from at least three independent experiments. and versus HG group.

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