GOLM1 as a Potential Therapeutic Target Modulates B7-H3 Secretion to Drive Ovarian Cancer Metastasis

Figure 2

B7-H3 expression is partially regulated by GOLM1. (a) qPCR analysis of GOLM1 knockdown efficiency in SKOV3 cell line after stable introduction of three different shRNAs by lentivirus system. (b) qPCR analysis of B7-H3 mRNA expression in three GOLM1 shRNA stable induced SKOV3 cell lines. (c) Western blot analysis of GOLM1 and B7-H3 protein expression in three GOLM1 knockdown SKOV3 cell lines. (d) Statistics of GOLM1 and B7-H3 protein expression in three GOLM1 knockdown SKOV3 cell lines. (e) qPCR analysis of GOLM1 and B7-H3 expression in SKOV3 cell line with transient exogenous expressed GOLM1 using PEI proreagent. (f) Western blot analysis of GOLM1 and B7-H3 expression in GOLM1 overexpressed SKOV3 cell line. (g) Statistics of GOLM1 and B7-H3 expression in GOLM1 overexpressed SKOV3 cell line. Each experiment was repeated three times and values are presented as means ± SEM (, two-tailed Student’s t-test).

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