GOLM1 as a Potential Therapeutic Target Modulates B7-H3 Secretion to Drive Ovarian Cancer Metastasis

Figure 3

GOLM1 expression was correlated with B7-H3 membrane protein expression and B7-H3 secretion. (a) FACS analysis of comparable B7-H3 membrane expression in GOLM1 knockdown and GOLM1 overexpressed SKOV3 cell lines. Original SKOV3 cell line as the control. (b) Statistics of B7-H3 membrane expression in three SKOV3 cell lines indicated in (a). (c) ELISA assay of soluble B7-H3 expression in three SKOV3 cell lines after cultured in incubator for three indicated times (24 hours, 48 hours, and 72 hours). Three independent experiments are performed, and values are presented as means ± SEM (, two-tailed Student’s t-test). (d) The interaction of exogenous expressed GOLM1 and B7-H3 was detected in 293T cells cotransfected with His-GOLM1 and Flag-B7-H3. (e) The interaction endogenously expressed GOLM1, and B7-H3 was detected in SKOV3 cells.

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