GOLM1 as a Potential Therapeutic Target Modulates B7-H3 Secretion to Drive Ovarian Cancer Metastasis

Figure 4

GOLM1 regulates ovarian cancer metastasis and invasion ability in soluble B7-H3-dependent manner. (a) Western blot of B7-H3, GOLM1 and metastasis-related protein E-cadherin, MMP-9, and VEGF in GOLM1 knockdown and GOLM1 overexpressed SKOV3 cell lines. Both scramble shRNA knockdown SKOV3 cell line and original SKOV3 cell line are treated as the control. (b) Western blot of lentivirus induced stable knockdown B7-H3 in SKOV3 cell lines compared with original and GOLM1 knockdown SKOV3 cell lines. (c) The Transwell assay to determine the invasiveness of GOLM1 knockdown and B7-H3 knockdown SKOV3 cell lines treated with or without additional soluble B7-H3 (10 μg/ml) for 48 hours. (d) Statistics of the invaded cell number between each group (). (e) The wound-healing ability of GOLM1 knockdown and B7-H3 knockdown SKOV3 cell lines treated with or without soluble B7-H3 (10 μg/ml) for 48 hours. (f) Statistics of the percentage of wound area remaining between each group ().

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