Therapeutic Effect and Mechanism of Negative Pressure Wound Therapy with Huoxue Shengji Decoction Instillation for Chronic Skin Ulcers

Table 1

RT-PCR conditions.

Stage	Temperature (°C)	Time (s)	Cycles
Pre-denaturation	95	30	1
PCR	95	5	40
	55	30
	72	34