Research Article

Proteomic Analyses Reveals the Mechanism of Acupotomy Intervention on the Treatment of Knee Osteoarthritis in Rabbits

Table 1

The information of differently accumulated proteins (fold change <0.7 or >1.3) in the acupotomy intervention (AI) group vs. the knee osteoarthritis (KOA) group.

AccessionProtein descriptionGene nameMW (kDa)Coverage (%)PeptidesRatio value

Energy metabolism
 G1T0U9Creatine kinase S-type, mitochondrialCKMT247.4538.70140.500.0124
 G1SZ233-Hydroxybutyrate dehydrogenase 1BDH137.6911.4040.580.0012
 G1SXI9Cytochrome c oxidase subunitCOX6B10.1747.7040.590.0147
 G1T4Q4Cytochrome c oxidase subunit 7A1COX7A18.8716.5010.630.0183
 G1TZN7Cytochrome c oxidase subunit 5ACOX5A20.4223.6030.650.0213
 G1TCZ0Cytochrome c oxidase subunit 5BCOX5B13.7624.8030.680.0475
 G1SG11Cytochrome c oxidase subunit 4 isoform 1, mitochondrialCOX4I119.5526.0040.690.0475
 U3KMU6Cytochrome b-c1 complex subunit Rieske, mitochondriaUQCRFS114.9510.4010.650.0283
 P98049Cytochrome c oxidase subunit 2MT-CO225.9715.9030.710.0231
 G1ST31Cytochrome c oxidase subunit 6A, mitochondrialCOX6A14.7217.3020.650.0439
 A0A5F9CAY2Cytochrome c oxidase subunit 4 isoform 1, mitochondrialCOX4I110.0420.5011.540.0016
 G1SDJ3ATP synthase membrane subunit DAPITDAPIT6.4225.9010.600.0311
 A0A5F9C153ATP synthase subunit O, mitochondrialATPeF0O25.7232.9060.730.0370
 G1SKT4ATP synthase subunit alphaATP5F1A59.7528.60140.750.0433
 G1T9N2ATP synthase subunit d, mitochondrial isoform X2ATPeF0D15.7424.8030.770.0376
 G1TES2ATPase inhibitor, mitochondrialATP5IF116.7712.8024.180.0054
 U3KNE2NDUFA4, mitochondrial complex-associatedNDUFA49.3337.8030.650.0078
 A0A5F9DDY7UQCRC2 isoform 4QCR244.5135.40100.680.0370
 G1SGP1Ubiquinol-cytochrome c reductase core protein 1UQCRC152.4131.90110.720.0237
 A0A5F9DAA7Thioredoxin domain-containing proteinPRDX330.1519.8050.730.0059
TCA cycle
 U3KMH9Malate dehydrogenaseMDH240.5337.00110.620.0322
 G1SKD9Succinate--CoA ligase subunit alpha, mitochondrialSUCLG136.2213.3040.630.0330
 G1U276Succinate--CoA ligase subunit beta, mitochondrialSUCLA258.4424.60120.640.0142
 A0A5F9CIE2Succinate dehydrogenase [ubiquinone] flavoprotein subunitSDH160.3315.0070.690.0231
 G1TA59Isocitrate dehydrogenase [NAD] subunit, mitochondrialIDH3A49.0411.0040.640.0137
 A0A5F9D9Z6Isocitrate dehydrogenase [NAD] subunit, mitochondrialIDH3B40.9614.4040.740.0108
 G1TUX2Aconitate hydratase, mitochondrialACO287.3533.10200.690.0388
 G1T9S4Acetyltransferase component of pyruvate dehydrogenase complexDLAT68.208.7050.710.0383
 A0A5F9DJY5Pyruvate dehydrogenase E1 alpha 1 subunitPDHA140.1729.20100.740.0299
 A0A5F9CUY0Oxoglutarate dehydrogenaseOGDH113.6123.80210.750.0206
 A0A5F9CAW7Dihydrolipoamide S-succinyltransferaseDLST48.5825.3080.760.0329
Amino acid metabolism
 G1SW97Isovaleryl-CoA dehydrogenaseIVD46.319.9040.690.0407
 A0A5F9DQB13-Hydroxyisobutyrate dehydrogenaseHIBADH34.275.8010.670.0250
 A0A5F9DH24Aminoadipate-semialdehyde synthaseAASS96.453.8030.740.0240
 G1SP323-Hydroxyisobutyryl-CoA hydrolase, mitochondrialHIBCH43.438.0030.750.0053
 P00008Cytochrome cCYCS11.6921.0030.620.0461
Lipid metabolism
 G1U891HADHB isoform 1HADHB49.7417.8070.680.0405
 G1SCE7HADHB isoform 1HADHB51.4519.2080.730.0124
 G1SPY9Acyl-CoA synthetase medium chain family member 5ACSM564.605.5030.730.0363
 G1SPZ9Pyrophosphatase (inorganic) 2PPA238.738.8020.760.0241
 G1SRD2KAT8 regulatory NSL complex subunit 1 likeKANSL1L44.0416.7050.710.0363
Protein metabolism
 A0A5F9CND9R3H domain-containing 1R3HDM1111.071.3010.740.0415
 G1SI29Elongation factor tuTUFM49.7117.8060.760.0325
 U3KLZ3Heterogeneous nuclear ribonucleoprotein A3HNRNPA137.679.0031.390.0310
Transportation
 G1SER3Solute carrier family 25 member 11SLC25A1134.0618.2050.620.0195
 G1T2J6Solute carrier family 25 member 12SLC25A1271.4614.3090.700.0459
 G1T237Solute carrier family 25 member 3SLC25A339.9013.0040.730.0326
 P12345Aspartate aminotransferase, mitochondrialGOT247.4123.3090.620.0463
 G1T332Aspartate aminotransferaseGOT146.4621.3080.630.0126
 G1SG30NipSnap homolog 2NIPSNAP230.6419.1040.640.0319
 O46373ADP/ATP translocase 1SLC25A432.9026.8080.680.0390
 P68003Voltage-dependentanion-selective channel protein 2VDAC231.5831.6070.720.0444
Cell development
 Q95MN6Proteolipid protein 2PLP216.758.6011.310.0130
 A0A5F9CPW4FibronectinFN1226.972.1031.370.0020
 G1SWS9VimentinVIM53.6544.40201.480.0219
 A0A5F9D4Y9Collagen alpha-1(XII) chainCOL12A1203.081.6031.590.0445
 G1T0G2Myosin binding protein HMYBPH54.3131.40131.600.0170
 A0A5F9DVP3Collagen type XIV alpha 1 chainCOL14A1181.2616.50231.640.0220
 A0A5F9CLG7Dihydropyrimidinase like 3DPYSL362.503.0021.960.0379
 G1SNE8S_100 domain-containing proteinS100A1021.9714.1031.360.0245
Signaling transduction
 A0A5F9CM55Myosin heavy chain 2MYH79.9450.60390.740.0484
 U3KP10Apoptosis inducing factor mitochondria associated 1AIFM166.1614.1070.660.0191
 A0A5F9DM36Protein-histidineN-methyltransferaseSETD366.553.7021.310.0192
 G1T2F2Peptidyl-prolylcis-trans isomerasePPIB24.066.0011.330.0311
 G1U0N7Olfactory receptor family 10 subfamily J member 3OR10J334.814.8011.430.0375
 A0A5F9CI92Reverse transcriptase domain-containing proteinCSMD45.893.2011.910.0491
Others
 G1TRK3Morphine-6-dehydrogenasePGER436.4822.9051.360.0246
 G1T6414HBT domain-containing proteinACOT1341.438.1030.660.0149