Evaluation of In Vivo Wound-Healing and Anti-Inflammatory Activities of Solvent Fractions of Fruits of Argemone mexicana L. (Papaveraceae)

Figure 4

Hematoxylin and eosin are used to stain the histopathology of the skin at day 18 (100 XS). (a-b) The black arrow indicates granulation tissue with many inflammatory cells (IC) and lacks collagen tissue and blood vessels in rats treated with simple ointment and 5% aqueous fraction. (c-d) The black arrow indicates granulation tissue in 5% ethyl acetate and n-hexane fraction treated rats with few inflammatory cells (IC), fibroblasts (F), collagen tissues (C), and less vascularized (BV). (e-g) The 10% ethyl acetate fraction and MEBO-treated rats' skin healed a well-formed, nearly normal epidermis, repaired adnexa, and a significant amount of fibrosis and collagen tissue in the dermis. (f-h) The 10% n-hexane and aqueous fraction treated rats also showed a significant fibrosis, accumulation of collagen tissues, and blood vessel formation or vascularization.