Ginsenoside Rh4 Inhibits Colorectal Cancer Cell Proliferation by Inducing Ferroptosis via Autophagy Activation

Figure 4

The inhibitory effect of the autophagy inhibitor 3-MA on ferroptosis induced by Rh4 in CRC cells. (a) The relative concentrations of GSH in HT29 and HCT116 cells exposed to Rh4-containing medium for 48 hours with or without 2 mM 3-MA pretreatment for 4 hours. (b) The iron concentrations in HT29 and HCT116 cells exposed to Rh4-containing medium for 48 hours with or without 2 mM 3-MA pretreatment for 4 hours. (c) The relative concentrations of lipid ROS in HT29 and HCT116 cells exposed to Rh4-containing medium for 48 hours with or without 2 mM 3-MA pretreatment for 4 hours. (d) The concentrations of MDA in HT29 and HCT116 cells exposed to Rh4-containing medium for 48 hours with or without 2 mM 3-MA pretreatment for 4 hours. (e) Representative Western blot images of ferroptosis-related protein expression in HT29 and HCT116 cells exposed to Rh4-containing medium for 48 hours with or without 2 mM 3-MA pretreatment for 4 hours. (f), (g) Relative expression levels of proteins involved in the ferroptosis pathway in HT29 and HCT116 cells exposed to Rh4-containing medium for 48 hours with or without 2 mM 3-MA pretreatment for 4 hours. , : statistically significant compared with the control group (0 μM Rh4); ^##, ^###: statistically significant compared with the 200 μM Rh4 group.

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