Ginsenoside Rh4 Inhibits Colorectal Cancer Cell Proliferation by Inducing Ferroptosis via Autophagy Activation

Figure 6

Rh4 can activate autophagy and induce ferroptosis in human CRC cells in vivo. Mice were treated with or without the Rh4. (a) Relative ROS concentrations in HT29 and HCT116 cell xenograft tumor tissues. (b) Relative GSH concentrations in HT29 and HCT116 cell xenograft tumor tissues. (c) Iron concentrations in HT29 and HCT116 cell xenograft tumor tissues. (d) MDA concentrations in HT29 and HCT116 cell xenograft tumor tissues. (e) Immunohistochemical staining was performed to analyze the expression levels of autophagy- and ferroptosis-related proteins in HT29 and HCT116 cell xenograft tumor tissues. Scale bars = 100 μm. (f) Western blotting was performed to analyze the expression level of autophagy-related proteins in HT29 and HCT116 cell xenograft tumor tissues. (g), (h) Relative expression levels of autophagy-related proteins in HT29 and HCT116 cell xenograft tumor tissues. (i) Western blotting was performed to analyze ferroptosis-related protein expression levels in HT29 and HCT116 cell xenograft tumor tissues. (j), (k) Relative expression levels of ferroptosis-related proteins in HT29 and HCT116 cell xenograft tumor tissues. , : statistically significant compared with the control group (solvent).

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