Research Article
Evaluation of Antimicrobial Activity, Cytotoxicity, and Phytochemical Composition of Ocimum americanum L. (Lamiaceae)
Table 2
Summary of mortality per test dose of Ocimum americanum L. crude extracts and fractions against brine shrimp larvae (Artemia salina nauplii).
| Sample | Mortality per test dose | LC50 µg/mL | 95% CI | Clarkson’s scale | 10 µg/mL | 100 µg/mL | 1000 µg/mL |
| Vincristine: positive control | 19 | 50 | 50 | 11.83 | – | Highly toxic | Acetone crude extract | 0 | 0 | 50 | 303.39a | 198.42–488.86 | Moderately toxic | Chloroform fraction of the acetone extract | 0 | 0 | 50 | 303.39a | 198.42–488.86 | Moderately toxic | Ethyl acetate fraction of the acetone extract | 0 | 0 | 50 | 303.39a | 198.42–488.86 | Moderately toxic | Aqueous crude extract | 0 | 3 | 35 | 559.71 | 397.09–811.09 | Slightly toxic | Chloroform fraction of the aqueous extract | 0 | 0 | 50 | 303.39b | 198.42–488.86 | Moderately toxic | Ethyl acetate fraction of the aqueous extract | 0 | 0 | 50 | 303.39b | 198.42–488.86 | Moderately toxic | Hydroethanolic crude extract | 0 | 0 | 49 | 347.80 | 217.06–498.10 | Moderately toxic | Chloroform fraction of the hydroethanolic crude extract | 49 | 50 | 50 | 0.59 | – | Highly toxic | Ethyl acetate fraction of the hydroethanolic crude extract | 0 | 48 | 50 | 44.65 | 4.65–65.86 | Highly toxic |
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The extracts and fractions denoted by a and b have no significant difference ( ) in between the groups and in the same group. |