Evaluation of Antimicrobial Activity, Cytotoxicity, and Phytochemical Composition of Ocimum americanum L. (Lamiaceae)

Table 2

Summary of mortality per test dose of Ocimum americanum L. crude extracts and fractions against brine shrimp larvae (Artemia salina nauplii).

Sample	Mortality per test dose			LC50 µg/mL	95% CI	Clarkson’s scale
	10 µg/mL	100 µg/mL	1000 µg/mL
Vincristine: positive control	19	50	50	11.83	–	Highly toxic
Acetone crude extract	0	0	50	303.39^a	198.42–488.86	Moderately toxic
Chloroform fraction of the acetone extract	0	0	50	303.39^a	198.42–488.86	Moderately toxic
Ethyl acetate fraction of the acetone extract	0	0	50	303.39^a	198.42–488.86	Moderately toxic
Aqueous crude extract	0	3	35	559.71	397.09–811.09	Slightly toxic
Chloroform fraction of the aqueous extract	0	0	50	303.39^b	198.42–488.86	Moderately toxic
Ethyl acetate fraction of the aqueous extract	0	0	50	303.39^b	198.42–488.86	Moderately toxic
Hydroethanolic crude extract	0	0	49	347.80	217.06–498.10	Moderately toxic
Chloroform fraction of the hydroethanolic crude extract	49	50	50	0.59	–	Highly toxic
Ethyl acetate fraction of the hydroethanolic crude extract	0	48	50	44.65	4.65–65.86	Highly toxic

The extracts and fractions denoted by _a and _b have no significant difference () in between the groups and in the same group.