Paeoniflorin Enhances the Sensitivity of ER-Positive Breast Cancer Cells to Tamoxifen through Promoting Sirtuin 4

Figure 2

Paeoniflorin enhances tamoxifen-induced ER-positive breast cancer cells apoptosis. (a and b) T47D and MCF-7 cells were treated with different concentrations of tamoxifen (1, 2, 4, 8, 16, 32, and 64 μM) for 48 hours, and the cell viability was determined using the CCK8 assay kit. Data were expressed as mean ± SD with 3 independent repetitions. ^ns, , , and vs solvent group. (c and d) T47D and MCF-7 cells were treated with or without PF (60 μM) or TAM (8 μM for T47D and 16 μM for MCF-7) for 48 hours, and the apoptosis of T47D and MCF-7 cells were analyzed using a flow cytometer. Data were expressed as mean ± SD with 3 independent repetitions. ^ns and indicated difference between two groups. (e) Representative flow cytometry scatter plot of cell apoptosis. PF = paeoniflorin; TAM = tamoxifen.

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