Paeoniflorin Enhances the Sensitivity of ER-Positive Breast Cancer Cells to Tamoxifen through Promoting Sirtuin 4

Figure 3

Effects of paeoniflorin on apoptosis-related protein expression after inducing with tamoxifen in ER-positive breast cancer cells. (a) Immunoblotting was used to detect the expression of Bax and Bcl2 in T47D cells after different treatments. (b) Statistically compare the ratio of BAX/Bcl2 protein expression in T47D cells of different groups. (c) The caspase 8 ELISA kit was used to detect the activity of caspase 8 in T47D cells of different groups. (d) Immunoblotting was used to detect the expression of Bax and Bcl2 in MCF-7 cells after different treatments. (e) Statistically compare the ratio of BAX/Bcl2 protein expression in MCF-7 cells of different groups. (f) The caspase 8 ELISA kit was used to detect the activity of caspase 8 in MCF-7 cells of different groups. Data were expressed as mean ± SD with 3 independent repetitions. ^ns and between two groups. PF = paeoniflorin; TAM = tamoxifen.

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