Rapid-Onset Antidepressant-Like Effect of Nelumbinis semen in Social Hierarchy Stress Model of Depression

Figure 7

Effects of Nelumbinis semen (NS) and AMPA blockade on GluR1 expression in the prefrontal cortex (PFC) and hippocampus. (a) Normalized GluR1/ß-actin of PFC: there was no significant change (F(3,20) = 1.145, ). (b) Normalized phospho-GluR1/ß-actin of PFC: there was no significant change (F(3,20) = 0.517, ). (c) Representative blot image of the total and phospho-GluR1 expression in the PFC. The parentheses correspond to the treatments indicated above them. (d) Normalized GluR1/ß-actin of the hippocampus: ANOVA revealed no significant differences among groups (F(3,20) = 2.692, ). (e) Normalized phospho-GluR1/ß-actin of the hippocampus: the phospho-GluR1 levels changed significantly (F(3,20) = 4.48, ). Phospho-GluR1 decreased with NS treatment (), but the difference between the NS and NS + NBQX groups was nonsignificant. (f) Representative blot image of the total and phospho-GluR1 expression in the hippocampus. The parentheses in (c) and (f) correspond to the treatments indicated above them. Data are shown as the mean ± SEM (n = 6). vs. vehicle, ^# vs. NS. Non-sig: nonsignificant. ANOVA, LSD post hoc test.

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