Exploring the Muscle Metabolomics in the Mouse Model of Sepsis-Induced Acquired Weakness

Figure 1

The C57BL/5 mice were classified into the following three groups: the no-treatment group (normal control group), the sepsis model group, and the sepsis-AW model group, with six mice in each group. The representative plots are shown as follows. (a) Mass variation diagram of the lower limb muscles and body weight of the normal control group, sepsis group, and sepsis-AW group. (b) The grip strength of the lower limb muscles computed using an electronic grip strength meter. (c) Mouse survival (n = 12 mice/group) were assessed by the log-rank test. (d) Cytokines level (IL-1β, IL-6, TNF-α) in the serum in mice were measured by the mouse cytokine ELISA kit at 5 d following modeling. (e) The morphological changes in the lower limb muscles of the mice in each group observed using transmission electron microscopy. (f, g) ATPase and COX staining of tibialis anterior muscles groups from normal control, sepsis, and sepsis-AW group.

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