Single-Cell RNA-Sequencing Reveals the Cellular and Genetic Heterogeneity of Skin Scar to Verify the Therapeutic Effects and Mechanism of Action of Dispel-Scar Ointment in Hypertrophic Scar Inhibition

Figure 9

(a) Immunofluorescence staining of TGF-β1 and Smad4 in skin tissues. TGF-β1 (red), Smad4 (green), and DAPI (blue). Bar = 100 μm. (b) Immunofluorescence staining of Col1a1 and MMP9 in skin tissues. Col1a1 (red), MMP9 (green), and DAPI (blue). Bar = 100 μm. (c) Quantitative analysis of TGF-β1-positive and Smad4-positive area density; n = 5 for each group. (d) Quantitative analysis of Col1a1- and MMP9-positive area density; n = 5 for each group. Significant differences between different groups or any other groups vs the model group are expressed as , , and , while significant differences between any other group and the control group are expressed as ^# = , ^## = , and ^### = .

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