Evidence-Based Complementary and Alternative Medicine

Research Article

Molecular Characterization of Gene-Mediated Resistance and Susceptibility of ESKAPE Clinical Isolates to Cistus monspeliensis L. and Cistus salviifolius L. Extracts

Primers for PCR amplification of gene-mediated resistance.


Genes	Primers	Sequences (5′–3′)	Products size (pb)	References

bla_CTX-M	CTX–M1–F CTX–M1–R	TTGGTGACGATTTTAGCCGC GGT TAA AAA ATC ACT GCG TC	864	[77]
bla_SHV	SHV–F SHV–R	TTATCTCCCTGTTAGCCACC GATTTGCTGATTTCGCTCGG	870	[78]
bla_TEM	TEM–F TEM–R	ATA AAA TTC TTG AAG ACG AAA GAC AGT TAC CAA TGC TTA ATC A	1080	[14]
bla_OXA	OXA–48–F OXA–48–R	GCTTGATCGCCCTCGATT GATTTGCTCCGTGGCCGAAA	281	[15]
bla_NDM	NDM–F NDM–R	GGTTTGGCGTCTGGTTTTC CGGAATGGCTCATCACGATC	621	[79]
bla_VIM	VIM–F VIM–R	GATGGTGTTTGGTCGCATA CGAATGCGCAGCACCAG	390	[79]
bla_OXA	OXA–51–F OXA–51–R	TAATGCTTTGATCGGCCTTG TGGATTGCACTTCATCTTGG	353	[80]
bla_OXA	OXA–23–F OXA–23–R	GATCGGATTGGAGAACCAGA ATTTCTGACCGCATTTCCAT	501	[80]
bla_OXA	OXA–58–F OXA–58–R	AAGTATTGGGGCTTGTGCTG CCCCTCTGCGCTCTACATAC	599	[80]
bla_IMP	IMP–F IMP–R	CTACCGCAGCAGAGTCTTTG AACCAGTTTTGCCTTACCAT	587	[81]
bla_VIM′	VIM′–F VIM′–R	GGTGTTTGGTCGCATATCGCAAC TGTGCTKGAGCAAKTCYAGACCG	390	[82]
bla_IMP′	IMP1–F IMP1–R	AGCAAGTTATCTGTATTCTT TTTRCTTTCDTTNARYCCTT	713	[83]
bla_NDM′	NDM′–F NDM′–R	AATGGAATTGCCCAATAT CGAAAGTCAGGCTGTGTT	489	[83]
bla_OXA′	OXA′–48–F OXA′–48–R	TTGGTGGCATCGATTATCGG GAGCACTTCTTTTGTGATGGC	744	[84]
bla_KPC′	KPC′–F KPC′–R	ATGTCACTGTATCGCCGTCT TTACTGCCCGTTGACGCCCA	881	[83]
bla_mecA	mec–A–F mec–A–R	GATATCGAGGCCCGTGGATT ACGTCGAACTTGAGCTGTTA	642	[85]
bla_VanA	van–A–F van–A–R	GGGAAAACGACAATTGC GTACAATGCGGCCGTTA	732	[16]