Molecular Characterization of Gene-Mediated Resistance and Susceptibility of ESKAPE Clinical Isolates to Cistus monspeliensis L. and Cistus salviifolius L. Extracts

Table 2

Amplification conditions of genes NDM, VIM, IMP, KPC, OXA-48, screened for P. aeruginosa.

Amplification steps	Temperature conditions/duration
	bla NDM	bla VIM	bla IMP	bla KPC	bla OXA-48
Initial denaturation	94°C/5 min
Denaturation	94°C/1 min
Annealing	57°C/1 min	60°C/1 min	50°C/1 min	60°C/1 min	58°C/1 min
Extension	72°C/1 min
Final elongation	72°C/7 min
Number of cycles	30