Molecular Characterization of Gene-Mediated Resistance and Susceptibility of ESKAPE Clinical Isolates to Cistus monspeliensis L. and Cistus salviifolius L. Extracts

Table 3

Amplification conditions of genes CTX-M, TEM, SHV, OXA-48, NDM, VIM, OXA-51, OXA-23, IMP, mecA, and VanA, screened for Enterobacteriaceae, A. baumannii, S. aureus, and E. faecium.

Amplification steps	Temperature conditions/duration
	bla CTX-M, SHV	bla TEM	bla OXA-48	bla NDM, VIM	bla OXA-51, OXA-21,OXA-58, IMP	bla mecA	bla VanA
Initial denaturation	94°C/5 min	94°C/5 min	94°C/10 min	94°C/10 min	95°C/2 min	95°C/1 min	94°C/2 min
Denaturation	94°C/1 min	94°C/1 min	94°C/30s	94°C/30 s	95°C/5 s	94°C/1 min	94°C/1 min
Annealing	60°C/1 min	42°C/1 min	55°C/1 min 30s	52°C/1 min 30s	60°C/10 s	52°C/1 min	54°C/1 min
Extension	72°C/1 min	72°C/1 min	72°C/1 min 30s	72°C/1 min 30s	72°C/10 s	72°C/1 min	72°C/1 min
Final elongation	72°C/10 min	72°C/10 min	72°C/10 min	72°C/10 min	72°C/10 s	72°C/10 min	72°C/10 min
Number of cycles	30	35	35	35	40	35	35