Antiplasmodial Activity of 80% Methanolic Extract and Solvent Fractions of Stem Bark of Acacia tortilis in Swiss Albino Mice

Table 4

Effects of crude extract and dichloromethane fraction on the parasitemia level and mean survival time in the curative test.

Groups	% Parasitemia					% Inhibition	Mean survival time
	D3	D4	D5	D6	D7
10 ml/kg DW	20.50 ± 0.76	22.67 ± 0.67	24.50 ± 0.56	26.17 ± 0.70	28.33 ± 0.67	0.00	8.00 ± 0.58
CHQ 25 mg/kg	21.67 ± 0.76	19.50 ± 0.67^a1,c3	13.33 ± 0.61^a3	5.17 ± 0.60^a3	0.00 ± 0.00^a3	100.00	30.00 ± 0.00^a3
100 mg/kg CE	22.67 ± 1.02	22.33 ± 0.80^d1,e2	22.00 ± 0.73^a1,b3	21.67 ± 0.84^a2,b3,e2	18.50 ± 0.89^a3,b3,e3	34.70	13.17 ± 0.60^a3,b3,e3
200 mg/kg CE	23.00 ± 1.13	22.17 ± 1.05	21.50 ± 0.89^a1,b3	20.17 ± 0.79^a3,b3,e1	16.17 ± 0.79^a3,b3,e3	42.93	15.17 ± 0.60^a3,b3,e1
400 mg/kg CE	22.50 ± 0.67	21.67 ± 0.56	19.33 ± 0.61^a3,b3	17.17 ± 0.54^a3,b3	10.17 ± 0.54^a3,b3	64.10	17.67 ± 0.42^a3,b3
10 ml/kg 2% T 80	20.00 ± 0.58	22.17 ± 0.79	23.83 ± 0.60	25.67 ± 0.84	27.17 ± 0.60	0.00	7.67 ± 0.33
CHQ 25 mg/kg	21.33 ± 0.76	19.00 ± 0.68^a1	12.67 ± 0.76^a3	4.83 ± 0.48^a3	0.00 ± 0.00^a3	100.00	30.00 ± 0.00^a3
100 mg/kg DF	22.50 ± 1.12	21.67 ± 0.92^d1,e2	21.50 ± 0.43^a2,b3,e2	20.67 ± 0.61^a3,b3,e3	18.33 ± 0.67^a3,b3,d3	32.54	13.33 ± 0.49^a3,b3,d3
200 mg/kg DF	22.00 ± 1.12	21.00 ± 0.77	20.17 ± 0.79^a2,b3	18.67 ± 0.80^a3,b3,e1	14.67 ± 0.71^a3,b3,e3	46.00	18.17 ± 0.79^a3,b3,e3
400 mg/kg DF	21.00 ± 0.68	19.50 ± 0.56	17.50 ± 0.76^a3,b3	15.17 ± 0.79^a3,b3	8.17 ± 0.48^a3,b3,c3	69.93	21.33 ± 0.42^a3,b3,c3

Data are expressed as mean ± SEM; n = 6, a = compared to the negative control, b = compared to the positive control, c = compared to 100 mg/kg dose, d = compared to 200 mg/kg dose, e = compared to 400 mg/kg dose, ¹, ², ³,  = negative and positive controls for dichloromethane fraction, D0 = day 0, D4 = day 4, DW = distilled water, CE = crude extract, DF = dichloromethane fraction, T 80 = tween 80, and CHQ = chloroquine.