Alpha-Mangostin Activates MOAP-1 Tumor Suppressor and Mitochondrial Signaling in MCF-7 Human Breast Cancer Cells

Figure 1

Cytotoxic effects of α-mangostin on cancer cell lines and spheroid cells. (a, b) Cytotoxicity of α-mangostin on human cell lines. The cell lines were treated with different concentrations of α-mangostin for 12 h (a) or extended up to 48 h (b) and the cell viability of the cells was measured to determine the IC50 values. (c) Selectivity index (SI) of α-mangostin. The cells were treated with the indicated concentration of α-mangostin, and the IC50 values of the treated cells were obtained. SI value of α-mangostin was determined in MCF-7 and MCF-7-CR as described in Methods section. (d) Time-dependent loss of cell viability of MCF-7 spheroid cells treated with α-mangostin. The cell viability of the spheroid cells was measured after treatment with α-mangostin at the indicated time. For control, the cells were treated with 1% DMSO (vol/vol) in the cell culture media, and the results from the control were used to normalize the data. (e) Morphology of α-mangostin-treated MCF-7 spheroid cells.

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