Alpha-Mangostin Activates MOAP-1 Tumor Suppressor and Mitochondrial Signaling in MCF-7 Human Breast Cancer Cells

Figure 2

α-Mangostin promotes apoptosis in MCF-7 cells. (a) α-Mangostin induced apoptotic cell death in MCF-7 cells with Annexin-V positive staining. MCF-7 cells were treated with different concentrations of α-mangostin followed by staining with Annexin-V and propidium iodide before flow cytometry analysis. (b) α-Mangostin induced apoptotic cell death in MCF-7 cells through the activation of caspase. The caspase activity of α-Mangostin-treated cells was measured using pGLO sensor −30F DEVD. (c) α-Mangostin induced nuclei condensation in MCF-7 cells. MCF-7 cells were treated with different concentrations of α-mangostin followed by DAPI staining and imaging using fluorescence microscopy. (d) Quantitative measurement of condensed nuclei in MCF-7 cells is described in (c).

(a)

(b)

(c)

(d)