Alpha-Mangostin Activates MOAP-1 Tumor Suppressor and Mitochondrial Signaling in MCF-7 Human Breast Cancer Cells

Figure 3

α-Mangostin induces the upregulation of endogenous MOAP-1 and promotes the interaction of MOAP-1 with activated BAX. (a) α-Mangostin induced the upregulation and the downregulation of endogenous MOAP-1 and BCL-XL, respectively. MCF-7 cells were treated with different concentrations of α-mangostin, and the cell lysates were prepared for immunoblot analysis using antibodies specific for each of the indicated proteins. (b) α-Mangostin induced the release of mitochondrial Cyt. (C) After treatment, the cytosol and mitochondria fractions were prepared for immunoblot analysis. (c) α-Mangostin promoted the interaction of MOAP-1 with activated BAX in MCF-7 cells. After treatment, the cell lysates were prepared for coimmunoprecipitation with N20 antibody specific for activated BAX (Act. BAX) followed by immunoblot analysis of the immunoprecipitants. (d) Oligomerization of activated BAX in MCF-7 cells treated with α-mangostin (30 µM). After treatment, the cell lysates were prepared for crosslinking with BMH followed by immunoblot analysis with an anti-BAX antibody. The protein bands were quantified using ImageJ [46].

(a)

(b)

(c)

(d)