Alpha-Mangostin Activates MOAP-1 Tumor Suppressor and Mitochondrial Signaling in MCF-7 Human Breast Cancer Cells

Figure 4

α-Mangostin enhances chemosensitization of MCF-7 cells and spheroids stably expressed HA-MOAP-1. (a) Western blot analysis of MCF-7 stable clones stably expressed HA-MOAP-1 or Myc-BCL-XL. The cell lysates of the stable clones were prepared and analyzed on a Western blot using the indicated antibodies. (b) The upregulation of HA-MOAP-1 and downregulation of Myc-BCL-XL proteins in the MCF-7 stable clones treated with α-mangostin. The cell lysates of the stable clones were prepared and analyzed with the indicated antibodies. α-Mangostin increased dose-dependent release of mitochondrial Cyt. C (c) and activation of caspase (d) of HA-MOAP-1 stable clone. The cytosolic and mitochondria fractions were prepared for immunoblot analysis with the indicated antibodies. The caspase activity of the treated cells was measured using pGLO sensor-30F DEVD. (e) Depletion of MCF-7 spheroid cells stably expressed HA-MOAP-1 by α-mangostin. Morphology of the spheroid MCF-7 cells treated with α-mangostin. (f) Quantification of the cell viability of the spheroid cells as described in (e). Cell viability of the spheroid cells was quantified at the indicated time after treatment with α-mangostin. The protein bands were quantified using ImageJ [46].

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