The Inhibitory Effect of Polyphenon 60 from Green Tea on Melanin and Tyrosinase in Zebrafish and A375 Human Melanoma Cells

Table1

The kinetic parameters of the L-DOPA activity of mushroom tyrosinase, in the presence of different concentrations of PP60.

Dose (µg/mL)	Vmax (ΔA/Sec)	km inhibition (mM) type	Ki (µg/mL)	Ki′ (µg/mL)
0.00	4.891 × 10^−6	0.043
0.25	4.848 × 10^−6	0.161
0.5	3.727 × 10^−6	0.181 mixed	1.125	11.35
1.0	3.599 × 10^−6	0.204
2.0	3.333 × 10^−6	0.235

V_max, Km, and Ki are equal to reaction velocity, Michaelis‒Menten constant, and El dissociation constant, respectively.