The Transcription Factor Otc4A Stimulates the Proliferation, Invasion, and Stemness of Colorectal Cancer Cells by Inhibiting the Regulation of miR-7-5p on TLR4

Figure 1

Effects of knockdown of Otc4A on the phenotype and stemness of colorectal cancer cells. qRT-PCR were used to detect Otc4A expression level in FHC and HT29 cells (a) and the si-NC and si-Otc4A groups (b); (c) CCK-8 for detecting the proliferation rate of cells; (d) cell cloning assay for the viability of cells; (e) Transwell for the invasion ability of cells; scale bar = 50 μm. (f) Sphere-forming assay for observing the sphere-forming ability of cells; scale bar = 100 μm. (g) Western blot for determining the expression level of Otc4A, p65, and p-p65. , . Each experiment was performed in triplicate.

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