The Transcription Factor Otc4A Stimulates the Proliferation, Invasion, and Stemness of Colorectal Cancer Cells by Inhibiting the Regulation of miR-7-5p on TLR4

Figure 3

Effects of miR-7-5p on TLR4 expression in colon cancer. (a) Database was utilized to predict the binding sites of miR-7-5p and TLR4; dual-luciferase reporter assay to verify the targeting relationship between miR-7-5p and TLR4; (b-d) qRT-PCR to check TLR4 expression level in FHC and HT29 cells (b) as well as miR-7-5p (c) and TLR4 (d) expression level in each group of cells; (e) Western blot to detect the protein level of TLR4 in cells; (f) correlation analysis for miR-7-5p and TLR4 expression; (g) qRT-PCR to observe the expression level of TLR4 in the si-NC and si-Otc4A groups; and (h) Correlation analysis for the association between Otc4A and TLR4 expression. , , . Each experiment was performed in triplicate.

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