The Transcription Factor Otc4A Stimulates the Proliferation, Invasion, and Stemness of Colorectal Cancer Cells by Inhibiting the Regulation of miR-7-5p on TLR4

Figure 4

Roles of Otc4A, miR-7-5p, and TLR4 in colorectal cancer. (a) CCK-8 was adopted to detect the proliferation rate of cells; (b) cell cloning assay to confirm the viability of cells; (c) Transwell assay to detect the invasion ability of cells; scale bar = 50 μm. (d) Sphere-forming assay to observe the sphere-forming ability of cells; scale bar = 100 μm. (e) Western blot to detect the expression level of TLR4, p65, and p-p65 in cells. vs. the si-NC group; , vs. the si-Otc4A group. Each experiment was performed in triplicate.

(a)

(b)

(c)

(d)

(e)