Cannabis Extract Effects on Metabolic Parameters and Gut Microbiota Composition in a Mice Model of NAFLD and Obesity
Table 4
Effect of cannabis extracts addition to obese mice during the consumption of an HFD on microbiota composition.
ND
HFD
HFD + CN1
HFD + CN2
HFD + CN6
α-Diversity
Pielou_e
0.90 ± 0.01
0.89 ± 0.01
0.91 ± 0.01€
0.88 ± 0.00$
0.91 ± 0.00#
Observed OUT’s
144.25 ± 2.39
108.40 ± 8.51
106.25 ± 9.75
89 ± 2.47,$
116.20 ± 10.09
Shannon index
6.43 ± 0.06
6.00 ± 0.09
6.09 ± 0.10,€
5.71 ± 0.05,$,#
6.22 ± 0.09
Phylum level
Actinobacteria
0.34 ± 0.17
0.17 ± 0.11
0.30 ± 0.14
0.18 ± 0.15$
0.69 ± 0.21#
Bacteroidetes
42.50 ± 1.92
67.16 ± 3.44
51.48 ± 7.13#,$,€
65.88 ± 3.82
66.49 ± 2.06
Deferribacteres
0.56 ± 0.18
1.81 ± 0.50
3.45 ± 1.26
3.14 ± 0.90
1.86 ± 0.37
Firmicutes
49.78 ± 2.18
21.14 ± 2.82
36.59 ± 6.38,#,€
24.83 ± 2.54
23.74 ± 1.01
Fusobacteria
1.65 ± 0.40
0.59 ± 0.25
2.01 ± 0.49#,€,$
0.84 ± 0.10
0.66 ± 0.28
Verrucomicrobia
0.18 ± 0.18
0.96 ± 0.10
0.66 ± 0.40
0.90 ± 0.41
1.17 ± 0.41
B/F ratio
0.87 ± 0.08
3.43 ± 0.51
1.75 ± 0.51#,€,$
2.85 ± 0.48
2.84 ± 0.22
Class level
Actinobacteria
0.33 ± 0.17
0.10 ± 0.04
0.16 ± 0.06€,$
0.15 ± 0.13
0.63 ± 0.19#
Bacteroidia
40.20 ± 1.64
66.40 ± 3.19
48.18 ± 7.88#,$,€
64.45 ± 3.80
65.71 ± 2.11
Flavobacteriia
2.30 ± 0.43
0.76 ± 0.44
3.30 ± 0.93#,$,€
1.40 ± 0.22
0.78 ± 0.35
Deferribacteres
0.56 ± 0.18
1.81 ± 0.50
3.45 ± 1.26
3.14 ± 0.90
1.86 ± 0.37
Bacilli
2.61 ± 0.54
1.19 ± 0.35
2.35 ± 0.56
1.74 ± 0.36
1.38 ± 0.26
Clostridia
45.94 ± 2.63
18.14 ± 3.10
32.30 ± 6.19,#,$,€
21.66 ± 2.22
20.26 ± 0.94
Fusobacteriia
1.65 ± 0.40
0.59 ± 0.25
2.01 ± 0.49#,$,€
0.84 ± 0.10
0.66 ± 0.28
Betaproteobacteria
0.46 ± 0.26
1.18 ± 0.45
1.06 ± 0.33
0.83 ± 0.23$
1.88 ± 0.43
Verrucomicrobiae
0.18 ± 0.18
0.96 ± 0.10
0.66 ± 0.40
0.90 ± 0.41
1.17 ± 0.41
Order level
Bifidobacteriales
0.33 ± 0.17
0.10 ± 0.04
0.16 ± 0.06$
0.15 ± 0.13$
0.63 ± 0.19#
Bacteroidales
40.20 ± 1.64
66.40 ± 3.19
48.18 ± 7.88#,$,€
64.45 ± 3.80
65.71 ± 2.11
Flavobacteriales
2.30 ± 0.43
0.76 ± 0.44
3.30 ± 0.93#,$,€
1.40 ± 0.22
0.78 ± 0.35
Deferribacterales
0.56 ± 0.18
1.81 ± 0.50
3.45 ± 1.26
3.14 ± 0.90
1.86 ± 0.37
Bacillales
1.64 ± 0.57
1.06 ± 0.31
2.04 ± 0.48$
1.50 ± 0.25
0.72 ± 0.32
Lactobacillales
0.97 ± 0.23
0.13 ± 0.09
0.31 ± 0.11
0.24 ± 0.20
0.66 ± 0.16#
Clostridiales
45.94 ± 2.63
18.14 ± 3.10
32.30 ± 6.19,#,$,€
21.66 ± 2.22
20.26 ± 0.94
Fusobacteriales
1.65 ± 0.40
0.59 ± 0.25
2.01 ± 0.49#,$,€
0.84 ± 0.10
0.66 ± 0.28
RF32
0.02 ± 0.01
0.14 ± 0.05
0.32 ± 0.12
0.25 ± 0.17
0.41 ± 0.20
Burkholderiales
0.44 ± 0.26
1.18 ± 0.45
1.06 ± 0.33
0.83 ± 0.23$
1.88 ± 0.43
RF39
0.3 ± 0.07
0.02 ± 0.02
0.00 ± 0.00
0.00 ± 0.00
0.01 ± 0.01
Verrucomicrobiales
0.18 ± 0.18
0.96 ± 0.10
0.66 ± 0.40
0.90 ± 0.41
1.17 ± 0.41
Family level
Bifidobacteriaceae
0.33 ± 0.17
0.10 ± 0.04
0.16 ± 0.06$
0.15 ± 0.13$
0.63 ± 0.19#
Bacteroidaceae
7.48 ± 1.42
43.58 ± 2.42
27.83 ± 5.38,#,€
45.35 ± 5.59
37.31 ± 4.24
Prevotellaceae
0.11 ± 0.04
0.21 ± 0.08
0.08 ± 0.04$
0.13 ± 0.05$
0.61 ± 0.17,#
Rikenellaceae
6.96 ± 0.60
3.19 ± 0.39
3.53 ± 1.03
3.63 ± 0.55
5.05 ± 0.86
S24-7
10.98 ± 1.17
8.98 ± 0.95
7.70 ± 1.31
5.87 ± 1.31,$
9.87 ± 0.50
Odoribacteraceae
1.65 ± 0.21
1.28 ± 0.44
1.31 ± 0.30
1.69 ± 0.24
2.41 ± 0.56
Flavobacteriaceae
2.30 ± 0.43
0.76 ± 0.44
3.30 ± 0.90#,$,€
1.40 ± 0.22
0.78 ± 0.35
Deferribacteraceae
0.56 ± 0.18
1.81 ± 0.5
3.45 ± 1.26
3.14 ± 0.90
1.86 ± 0.37
Bacillaceae
1.63 ± 0.57
1.06 ± 0.31
2.02 ± 0.48$
1.50 ± 0.25
0.72 ± 0.32
Lactobacillaceae
0.97 ± 0.23
0.13 ± 0.09
0.31 ± 0.11
0.23 ± 0.20
0.66 ± 0.16#
Christensenellaceae
0.75 ± 0.11
0.28 ± 0.08
0.39 ± 0.13
0.31 ± 0.13
0.29 ± 0.05
Clostridiaceae
0.94 ± 0.07
0.29 ± 0.19
0.46 ± 0.21
0.52 ± 0.24
0.28 ± 0.09
Dehalobacteriaceae
0.54 ± 0.07
0.63 ± 0.11
0.87 ± 0.17,$,€
0.45 ± 0.08
0.38 ± 0.08
Lachnospiraceae
5.87 ± 1.35
4.00 ± 0.54
7.66 ± 1.10#,€
3.05 ± 0.60
5.21 ± 0.45
Peptococcaceae
0.69 ± 0.12
0.11 ± 0.08
0.24 ± 0.15
0.06 ± 0.03
0.35 ± 0.21
Ruminococcaceae
18.76 ± 1.92
7.30 ± 1.23
10.80 ± 2.25,$
10.15 ± 0.56
6.21 ± 0.35
Fusobacteriaceae
1.65 ± 0.40
0.59 ± 0.25
2.01 ± 0.49#,$,€
0.84 ± 0.10
0.66 ± 0.28
Alcaligenaceae
0.37 ± 0.28
1.14 ± 0.43
0.85 ± 0.34
0.80 ± 0.22$
1.88 ± 0.43
Verrucomicrobiaceae
0.18 ± 0.18
0.96 ± 0.10
0.66 ± 0.40
0.90 ± 0.41
1.17 ± 0.41
Genus level
Bifidobacterium
0.33 ± 0.17
0.10 ± 0.04
0.16 ± 0.06$
0.15 ± 0.13$
0.63 ± 0.19#
Bacteroides
7.48 ± 1.42
43.58 ± 2.42
27.83 ± 5.38,#,€
45.35 ± 5.59
37.31 ± 4.24
Prevotella
0.11 ± 0.04
0.21 ± 0.08
0.08 ± 0.04$
0.13 ± 0.05$
0.61 ± 0.17,#
Butyricimonas
0.55 ± 0.17
1.06 ± 0.39
0.89 ± 0.25
1.37 ± 0.28
1.87 ± 0.49
Odoribacter
1.10 ± 0.37
0.22 ± 0.10
0.43 ± 0.22
0.32 ± 0.22
0.54 ± 0.20
Mucispirillum
0.56 ± 0.18
1.81 ± 0.50
3.45 ± 1.26
3.14 ± 0.90
1.86 ± 0.37
Lactobacillus
0.97 ± 0.23
0.13 ± 0.09
0.31 ± 0.11
0.23 ± 0.20
0.66 ± 0.16#
Dehalobacterium
0.54 ± 0.07
0.63 ± 0.11
0.87 ± 0.17,$,€
0.45 ± 0.08
0.38 ± 0.08
Coprococcus
0.70 ± 0.21
0.21 ± 0.12
0.31 ± 0.13
0.20 ± 0.06
0.22 ± 0.06
Roseburia
0.00 ± 0.00
0.08 ± 0.08
1.33 ± 0.72,#,€
0.09 ± 0.09
0.63 ± 0.11
Oscillospira
6.37 ± 0.70
1.86 ± 0.41
3.58 ± 1.33
3.71 ± 0.37
1.72 ± 0.16
Ruminococcus
1.14 ± 0.40
0.42 ± 0.20
1.12 ± 0.33
1.43 ± 0.23#,$
0.52 ± 0.22
Cetobacterium
1.65 ± 0.40
0.59 ± 0.25
2.01 ± 0.49#,$,€
0.84 ± 0.10
0.66 ± 0.28
Sutterella
0.37 ± 0.28
1.14 ± 0.43
0.83 ± 0.35$
0.80 ± 0.22$
1.88 ± 0.43
Akkermansia
0.18 ± 0.18
0.96 ± 0.10
0.66 ± 0.40
0.90 ± 0.41
1.17 ± 0.41
Species level
distasonis
0.00 ± 0.00
2.91 ± 0.43
1.96 ± 1.04
1.54 ± 1.54#
1.80 ± 0.51
schaedleri
0.56 ± 0.18
1.81 ± 0.50
3.45 ± 1.26
3.14 ± 0.90
1.86 ± 0.37
gnavus
1.27 ± 0.46
0.84 ± 0.30
1.87 ± 0.51€
0.51 ± 0.31
1.12 ± 0.20
flavefaciens
0.47 ± 0.23
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
somerae
1.65 ± 0.40
0.59 ± 0.25
2.01 ± 0.49#,$,€
0.84 ± 0.10
0.66 ± 0.28
muciniphila
0.18 ± 0.18
0.96 ± 0.10
0.66 ± 0.40
0.90 ± 0.41
1.17 ± 0.41
The effect of diets on gut microbiota richness and diversity as well as microbiota composition at all taxonomic levels. Male C57BL/6 J mice were fed with a high-fat diet supplemented with CN1 or CN2 or CN6 extracts 5 mg/kg BW for 8 weeks. Values are expressed as mean ± SEM (n = 5). versus ND group; # versus HFD; $ versus CN6 group; and € versus CN2 group.