Root Extract of a Micropropagated Prunus africana Medicinal Plant Induced Apoptosis in Human Prostate Cancer Cells (PC-3) via Caspase-3 Activation

Table 1

Phytochemical components detected in the methanol extract of P. africana experimental materials by GC/MS analysis. t_R: retention time (min); %: percent of total.

No.	Identified compound	PWS		PIS		PIR		PIL		PC
			%2	tR	%	tR	%	tR	%	tR	%
1	3-Furanmethanol	5.96	0.39	—	—	—	—	5.97	0.16	5.97	0.79
2	Dihydroxyacetone	7.01	2.22	6.96	0.72	—	—	7.01	0.62	7.10	1.31
3	Benzoic acid, methyl ester	12.83	0.46	—	—	—	—	8.11	0.35	—	—
4	4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl-	13.95	1.13	13.95	0.63	13.94	0.66	9.19	0.06	14.00	2.87
5	Benzoic acid	14.68	14.02	14.60	3.10	14.55	1.83	12.83	0.15	14.48	0.71
6	Catechol	15.17	4.74	—	—	15.17	0.32	13.97	0.80	—	—
7	4-Vinylphenol	15.61	0.77	15.62	0.65	15.63	0.80	14.99	6.79	—	—
8	5-Hydroxymethyl-2-furaldhyde	15.81	0.31	15.81	0.81	15.81	0.11	15.20	0.80	16.03	24.79
9	Isosorbide	17.10	0.98	—	—	—	—	15.62	1.66	—	—
10	Phenol, 2,6-dimethoxy-	18.39	0.41	—	—	18.39	0.04	15.83	0.32	—	—
11	4-Hydroxy-3-methoxybenzaldehyde	19.30	0.41	25.03	0.14	—	—	—	—	—	—
12	3,4-Altrosan	20.84	3.30	—	—	—	—	—	—	—	—
13	Mandelamide	21.54	0.40	—	—	—	—	21.64	1.41	—	—
14	Vanillic acid	22.13	0.93	—	—	22.12	0.78	—	—	—	—
15	Benzenepropanol, 4-hydroxy-3-methoxy-	23.61	2.14	—	—	—	—	—	—	—	—
16	Benzaldehyde, 4-hydroxy-3,5-dimethoxy-	23.83	0.75	—	—	—	—	—	—	—	—
17	4-(Hydroxymethyl)-2,6-dimethoxyphenol	24.57	0.17	—	—	—	—	—	—	—	—
18	(E)-4-(3-Hydroxyprop-1-en-1-yl)-2-methoxyphenol	25.03	0.18	25.03	0.14	25.03	0.10	25.05	0.38	—	—
19	6-Hydroxy-5-trifluoromethylcyclohexa-1,3-diene	25.98	3.65	—	—	—	—	—	—	—	—
20	Benzoic acid, 4-hydroxy-3,5-dimethoxy-	26.13	0.29	26.12	0.08	26.20	0.13	—	—	—	—
21	Isopropyl myristate	26.31	0.40	26.31	0.15	—	—	—	—	—	—
22	Sorbitol	27.95	0.05	27.00	0.09	29.62	5.75	29.82	2.88	—	—
23	n-Hexadecanoic acid	28.21	4.95	28.22	2.34	28.22	3.72	28.24	1.52	28.23	3.18
24	9,12-Octadecadienoic acid (Z, Z)-	30.51	0.20	30.52	0.79	30.52	0.64	30.57	0.88	30.52	0.21
25	Oleic acid	30.58	0.76	30.60	0.99	—	—	—	—	30.60	1.72
26	Octadecanoic acid	30.87	0.47	30.87	0.66	—	—	30.89	0.58	30.88	0.69
27	Benzyl, beta-d-glucoside	31.90	0.32	31.90	0.17	—	—	32.05	0.28	31.97	0.16
28	9-Octadecenamide, (Z)-	33.35	1.95	33.35	0.80	33.35	1.05	33.36	0.33	33.35	0.59
29	(R)-alpha-(beta-D-glucopyranosyloxy)benzene-acetonitrile	35.10	6.60	—	—	35.10	1.91	—	—	—	—
30	13-Docosenamide, (Z)-	37.88	6.49	37.88	6.76	37.88	4.53	37.88	0.93	37.87	1.07
31	Squalene	38.38	1.09	—	—	—	—	38.40	5.34	—	—
32	Beta-sitosterol	43.55	8.37	43.57	16.77	43.56	7.75	43.56	4.76	43.55	4.02