Study on the Mechanism of Bu-Shen-He-Mai Granules in Improving Renal Damage of Ageing Spontaneously Hypertensive Rats by Regulating Th17 Cell/Tregs Balance

Figure 6

Immunoregulation of BSHM was expanded by combined treatment with S6281 and offset by combined treatment with SR0987. (a) Representative pictures of HE staining (× 200) of spleens (n = 3). (b) The levels of plasma IL-6, IL-10, and IL-17A (n = 10). (c) Flow cytometry used to determine the levels of both Th17 cells and Tregs in peripheral blood (n = 3). (d) Representative pictures of flow cytometry (n = 3). (e) Representative pictures of immunofluorescence staining. Red area for Foxp3 and green area for RORγt. (n = 3). (f) The positive rates of immunofluorescence staining were analyzed by ImageJ (n = 3). Notes: vs. WKY group; ^△ vs. SHR group; ^△△ vs. SHR group; ^∇∇vs. BSHM group; vs. BSHM group.

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