Total Saponins of Radix Clematis Regulate Fibroblast-Like Synoviocyte Proliferation in Rheumatoid Arthritis via the LncRNA OIP5-AS1/MiR-410-3p/Wnt7b Signaling Pathway

Table 2

Primers used for real-time PCR.

Gene	Primers
LncRNA OIP5-AS1	Forward: 5′-AGTGCTGCGTTTTCTGCCCT-3′
	Reverse: 5′-CACCTCATACACTGGTGGCCC-3′
miR-410-3p	Forward: 5′-AATATAACACAGATGGCCTGT-3
	Downstream primers are directly provided by miRNA tailing kit
Wnt7b	Forward: 5′-GCGGTGATGGCGTATGT-3′
	Reverse: 5′-GGGCGCAGATGGGTATC-3′
β-Catenin	Forward: 5′-TATGAGTGGGAGCAAGGC-3′
	Reverse: 5′-CTGCGTGGATGGGATCT-3′
C-Myc	Forward: 5′-GGTCATCCCCATCAAGAG-3′
	Reverse: 5′-CATTTGCGGTTGTTGCT-3′
CyclinD1	Forward: 5′-GCGTACCCTGACACCAAT-3′
	Reverse: 5′-CTTCGCACTTCTGCTCCT-3′
GSK-3β	Forward: 5′-CTTTTCACAGGGCTACGC-3′
	Reverse: 5′-ACAGGCAAGCACATTTCC-3′
SFRP4	Forward: 5′-CCACACACCACTTGCCTT-3′
	Reverse: 5′-CACAGGCCACTCTGAACAC-3′
β-Actin	Forward: 5′-CCTCACTGTCCACCTTCCA-3′
	Reverse: 5′-GGGTGTAAAACGCAGCTCA-3′