Research Article
The Effects of Qinghao-Kushen and Its Active Compounds on the Biological Characteristics of Liver Cancer Cells
Figure 6
Matrine and scopoletin influenced the level of GSK-3β. (a) Matrine and scopoletin inhibited the activity of HepG2 and HepG2215 cells. We detected cell numbers at 12-, 24-, and 36-h treatment with different concentrations of drugs, respectively. The bar chart shows the effect of matrine and scopoletin on HepG2 and HepG2215 cell activity. (mean ± SD, n = 3). The IC50 of drugs was measured at 12 h, 24 h, and 36 h, respectively. (b) Matrine-scopoletin inhibited the activity of HepG2 and HepG2215 cells. (c) Matrine and scopoletin inhibited the migration capability of HepG2 and HepG2215 cells. Cells were treated with different drugs. (d) The expression of GSK-3β was detected with western blot assay. HepG2 and HepG2215 cells were treated with different drugs, and each treatment factor was repeated three times. The GAPDH blot served as the loading control. DMSO, dimethyl sulfoxide; Ma, matrine; Sc, scopoletin; Co, matrine-scopoletin; DDP, 5.0 μM cisplatin treatment. , , .
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