A Herbal Mixture of Sesami Semen Nigrum and Longan Arillus Induces Neurite Outgrowth in Cultured Neurons and Shows Anti-Depression in Chronic Mild Stress-Induced Rats

Figure 1

Herbal mixture of Sesami Semen Nigrum and Longan Arillus induces neuronal differentiation. PC12 cells were seeded onto a 6-well plate and cultured for 24 h, then the medium was replaced with low DMEM with 1% FBS and 1% HS in the presence of Sesami Semen Nigrum (SSN) and/or Longan Arillus (LA) or NGF. Fresh medium with drug was changed every 24 h. Images were taken after 3 days of treatment, and representative pictures are shown (upper panel). The percentage of differentiated cells was calculated and plotted (lower panel). Values are expressed in percentage of total number of cells, in mean ± SEM, n = 4. () .