Research Article
Edible Bird’s Nest Regulates Hepatic Cholesterol Metabolism through Transcriptional Regulation of Cholesterol Related Genes
Table 1
Names and primer sequences used in the study.
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aHousekeeping genes. bNormalization gene. Underlined sequences are universal left and right sequences (tags). cInternal control was supplied by Beckman Coulter Inc (Miami, FL, USA) as part of the GeXP kit. RT conditions were as follows: 48°C for 1 min, 37°C for 5 min, 42°C for 60 min, 95°C for 5 min, and then maintained at 4°C. PCR conditions were initial denaturation at 95°C for 10 min, followed by two-step cycles of 94°C for 30 s and 55°C for 30 s, ending in a single extension cycle of 68°C for 1 min. B2m: beta-2-microglobulin; Hprt1: hypoxanthine phosphoribosyltransferase 1; KanR: kanamycin resistant; Rpl13a: ribosomal protein L13a; CYP7A1: cholesterol 7 alpha-hydroxylase; LDLR: low-density lipoprotein receptor; PCSK9: proprotein convertase subtilisin/kexin type 9; ApoB: apolipoprotein B; HMGCR: HMG-CoA reductase/3-hydroxy-3-methyl-glutaryl-CoA reductase. |