Edible Bird’s Nest Regulates Hepatic Cholesterol Metabolism through Transcriptional Regulation of Cholesterol Related Genes

Table 1

Names and primer sequences used in the study.

Name	Left sequence	Right sequence
B2m^a	AGGTGACACTATAGAATAATGCTTGCAGAGTTAAACA	GTACGACTCACTATAGGGATGCATAAAATATTTAAGGTAAGA
Hprt1^a,b	AGGTGACACTATAGAATATCCTCATGGACTGATTATG	GTACGACTCACTATAGGGACTGGTCATTACAGTAGCTCTT
Rpl13a^a	AGGTGACACTATAGAATAATGGGATCCCTCCAC	GTACGACTCACTATAGGGAATTTTCTTCTCCACATTCTT
Kan(r)^c
CYP7A1	AGGTGACACTATAGAATAATAACATCCGAAAAGATGAC	GTACGACTCACTATAGGGATTTCCATTACTGTAGAAGGTG
LDLR	AGGTGACACTATAGAATAATGAGGTACGTAAGATGACC	GTACGACTCACTATAGGGAAGGTCAGACCAGTAAATTCT
PCSK9	AGGTGACACTATAGAATATGATTAATGCAGATCAGTTC	GTACGACTCACTATAGGGATTTATGTCCTAGCATCACTG
ApoB	AGGTGACACTATAGAATAATATCCTGAACATCAAGAGG	GTACGACTCACTATAGGGAGATGCTGTTCATTACAGATG
HMGCR	AGGTGACACTATAGAATATCATTCATTTCCTCGACA	GTACGACTCACTATAGGGATCTCCCTTACTTCATCCTGT

^aHousekeeping genes. ^bNormalization gene. Underlined sequences are universal left and right sequences (tags). ^cInternal control was supplied by Beckman Coulter Inc (Miami, FL, USA) as part of the GeXP kit. RT conditions were as follows: 48°C for 1 min, 37°C for 5 min, 42°C for 60 min, 95°C for 5 min, and then maintained at 4°C. PCR conditions were initial denaturation at 95°C for 10 min, followed by two-step cycles of 94°C for 30 s and 55°C for 30 s, ending in a single extension cycle of 68°C for 1 min. B2m: beta-2-microglobulin; Hprt1: hypoxanthine phosphoribosyltransferase 1; KanR: kanamycin resistant; Rpl13a: ribosomal protein L13a; CYP7A1: cholesterol 7 alpha-hydroxylase; LDLR: low-density lipoprotein receptor; PCSK9: proprotein convertase subtilisin/kexin type 9; ApoB: apolipoprotein B; HMGCR: HMG-CoA reductase/3-hydroxy-3-methyl-glutaryl-CoA reductase.