Sanzi Yangqin Decoction Alleviates Allergic Asthma by Modulating Th1/Th2 Balance: Coupling Network Pharmacology with Biochemical Pharmacology

Figure 6

SYD reduced the expression of IL-4 and IFN-γ in the lung tissue of the mice with asthma. (a) The gene expression of the core targets was detected by Q-PCR. (b and c) Protein expression of IL-4 and IFN-γ in the lung tissues were detected by western blot. β-actin served as a reference gene. All experiments were repeated in triplicate to average. (d and e) ELISA was used to measure the total IL-4 and IFN-γ content (pg/mL) in BALF of mice. (f) ELISA was used to measure the total IgE content (ng/mL) in serum of mice (blank, n = 10; model, n = 7; SYD 15 g/kg, n = 8; SYD 30 g/kg, n = 8; SYD 45 g/kg, n = 9; dexamethasone, n = 9).

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