[Retracted] Knockdown of lncRNA HOXD-AS2 Improves the Prognosis of Glioma Patients by Inhibiting the Proliferation and Migration of Glioma Cells

Figure 3

Levels of expression of HOXD-AS2 in U251 cell lines after siRNA transfection. Knockdown of HOXD-AS2 inhibits U251 cell growth. (a) The levels of expression of HOXD-AS2 in three glioma cell lines were analyzed by RT-qPCR. (b) The levels of expression of HOXD-AS2 in the U251 cell line were analyzed by RT-PCR; the relative gene expression level of SiHOXD-AS2-1—48 hr, SiHOXD-AS2-2—48 hr, and SiHOXD-AS2-3—48 hr was 0.20 ± 0.04, 0.34 ± 0.09, and 0.83 ± 0.12. NC, negative control; . (c) Knockdown of HOXD-AS2 reduced the growth of U251 cells compared with the NC group in the CCK-8 assay. (d) U251 cells were transfected with siRNAs, and after 48 h, cells were stained and imaged under a fluorescence microscope. Scale bars represent 50 μm. (e) The ratio of EdU‐positive cells was calculated and plotted on the histogram. (f) Cell cycle profiling was analyzed using flow cytometry. The image shows the flow cytometry distribution of each cycle in the three groups of cells 48 h after transfection. (g) The proportion of cells in G1 phase in interference groups 1 and 2 was higher than that in the NC group, indicating that the number of cells in S phase and G2 phase was reduced significantly. Data were based on at least three independent experiments and shown as mean ± SD. ; .

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