Antidiarrheal Effect of 80% Methanol Extract and Fractions of the Roasted Seed of Coffea arabica Linn (Rubiaceae) in Swiss Albino Mice

Table 5

In vivo antidiarrheal indices (ADIs) of 80% methanol crude extract and solvent fractions of roasted seed of Coffea arabica Linn.

Group	D freq (from castor oil-induced diarrhea)	meq (from charcoal meal test)	P freq (from castor oil-induced diarrhea)	ADI
Control	—	—	—	—
80ME 100	36.67	7.3	16.24	16.32
80ME 200	51.82	8.8	38.68	26.03
80ME 400	69.1	20.6	45.26	40.09
Posit con	162.42	53.5	74.27	86.42
Control	—	—	—	—
HF 100	23.16	8.6	15.6	14.6
HF 200	35.05	12.7	21.8	21.33
HF 400	41.76	20.4	28.1	28.82
Posit con	166.14	57	75	89.22
Control	—	—	—	—
EF 100	121.84	20.1	33.4	43.41
EF 200	128.74	37.9	43.4	59.6
EF 400	129.88	45	50	66.36
Posit con	154.88	61.6	70	87.41
Control	—	—	—	—
AF 100	40	9.1	10.4	15.59
AF 200	52.47	9.4	27.5	23.85
AF 400	66.38	15.9	31	31.98
Posit con	160.87	52	72.5	84.65

80ME = 80% hydromethanolic extract, HF = hexane fraction, EF = ethyl acetate fraction, and AF = aqueous fraction. Posit con is atropine 1 mg/kg for gastrointestinal motility test and loperamide 3 mg/kg in case of castor oil-induced diarrhea and enteropooling models. Controls received 10 ml/kg distilled water (for AF and 80ME) and 2% tween 80 (for EF and HF). D freq, the delay in diarrhea onset obtained from castor oil diarrhea test; meq, the gut meal motility reduction as % inhibition; P freq, the purging frequency or reduction in the number of wet stools as % inhibition of diarrhea; ADI, antidiarrheal index.