The Immunoregulatory Effect of Aconite Treatment on H22 Tumor-Bearing Mice via Modulating Adaptive Immunity and Natural Killer-Related Immunity

Table 1

Chemical compounds in aconite extract as analyzed by ultra-performance liquid chromatography high-resolution mass spectrometry.

Note	Retention time (min)	Chemical compounds	Molecular formula	Molecular weight
1	3.56	Isotalatizidine	C23H37NO5	407.54362
2	4.12	Songorine	C22H31NO3	357.48648
3	4.68	Hokbusine B	C27H35NO5	453.57054
4	5.10	Neoline	C24H39NO6	437.5696
5	6.00	Talatisamine	C24H39NO5	421.5702
6	7.81	14-Acetyltalatisamine	C26H41NO6	463.60688
7	9.87	Benzoylmesaconine	C31H43NO10	589.67386
8	10.83	Benzoylaconitine	C32H45NO10	603.70044
9	11.41	Benzoylhypacoitine	C31H43NO9	573.67446
10	12.25	Aconitine	C34H47NO11	645.73712
11	13.04	Hypaconitine	C34H47NO10	629.73772

The concentrations of safflomin A, benzoylmesaconine, benzoylaconitine, benzoylhypacoitine, aconitine, and hypaconitine were detected by the UHPLC-HRMS method and were 4.1 mg/g, 5.63 mg/g, 6.45 mg/g, 1.22 mg/g, and 1.35 mg/g, respectively, in the extracts. The chromatographic profile of the extract is shown in Figure 1.