Qinggan Huoxue Recipe Protects against Experimental Alcoholic Liver Fibrosis through CXCL16 Inhibition

Figure 9

QGHXR inhibits TLR4 and p-NF-κB expression in vitro and in vivo. (a) The cells stimulated by 100 ng/ml LPS were treated with different concentrations of QGHXR for 24 h. The mRNA level of CXCL16 was determined by real-time PCR. (b) The level of CXCL16 in the cell culture supernatant was determined by ELISA assay. (c) The levels of TLR4, p-NF-κB, and NF-κB protein expression in cells were determined by western blot. (d) The levels of TLR4, p-NF-κB, and NF-κB protein expression in the ethanol plus CCl₄-administrated mice liver were determined by western blot. The arbitrary units were analyzed by ImageJ software. The values represent means ± SEM. , vs. control.

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