Xuanhuang Runtong Tablets Relieve Slow Transit Constipation in Mice by Regulating TLR5/IL-17A Signaling Mediated by Gut Microbes

Figure 8

XHRT regulated the expression of TLR5/IL-17A related to the intestinal mucosal immune function in the STC mice induced by loperamide hydrochloride (a).Immunofluorescence method used antibodies targeting TLR5 and IL-17A proteins, observed the protein localization and fluorescence intensity under a laser confocal microscope, and used the average fluorescence intensity of the protein to reflect the relative expression level of each protein (b) (400×). (c) The relative expression of TLR5 mRNA and IL-17A mRNA in colon tissue by real-time fluorescent quantitative PCR with GAPDH as an internal reference was detected. STC: slow transit constipation; XHRT: Xuanhuang Runtong tablet; TLR5: toll receptor 5; IL-17A: interleukin-17A; GAPDH: glyceraldehyde 3-phosphate dehydrogenase. Values were expressed as the mean ± SD, vs. control group; , vs. STC model group.

(a)

(b)

(c)