Anti-Inflammatory Effects of Clematis terniflora Leaf on Lipopolysaccharide-Induced Acute Lung Injury

Figure 6

Effects of EELCT on inflammatory mediator production and pulmonary edema in ALI. Mice were intratracheally injected with LPS (5 mg/kg) before oral administration of EELCT or Dex. After 24 h, lung tissue and BALF were collected. (a–c) TNF-α, IL-6, and IL-1β levels in BALF were measured by ELISA. (d) NF-κB, ERK, COX-2, and iNOS expressions in lung tissues were investigated using Western blot. (e) Total protein concentrations in BALF were determined using the Bradford assay kit. (f) Graph shows lung/body weight ratios. (g) Mice were weighed before EELCT or Dex administration and before sacrifice. The graph reveals the body weight change. (h) The graph represents spleen/body weight ratios. Graph data represent mean ± SEM. compared with the ALI group. β-Actin was the loading control.

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