Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus

Figure 4

Thermostability of Tth α. Gap-filling polymerase assays [31] were used to determine loss of activity of Tth α at elevated temperatures. (a) The enzyme mix was heated to 90°C for 2 min and then combined with the substrate mix and incubated for an additional 5 min at 60°C. The positive control was not subjected to the heat challenge step. (b) Components of the complete Tth holoenzyme were mixed at room temperature and incubated at 80°C () or 85°C (). Samples were removed at the indicated times.

(a)

(b)