Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus

Figure 6

A comparison of the activity of Tth pol III holoenzyme at 55°C versus 98°C. Assays containing the optimized buffer system were cycled 5 times at 98°C/20 s, 60°C/2 min, and 70°C/2 min prior to addition of the dNTP mix and then incubation was continued at 70°C for an additional 2 min. Positive control contained the original buffer mix and was cycled as for optimized buffer system reactions but all cycle temperatures were at 55°C. Negative controls were cycled as for the optimized buffer system reactions but contained the original buffer mix.